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. 2017 Oct 2;114(42):E8930–E8939. doi: 10.1073/pnas.1710885114

Table S1.

Oligonucleotide primers used in this work

Assays Destination products Template Primer name Primer sequence (5′-3′)
Y2H AD-MED25 MED25 AD-MED25 F CGGAATTCATGTCGTCGGAGGTGAAACAGCTGATCGT
AD-MED25 R TTGGATCCCTCCCATGAAGCCAGCTCCAGGCATGTTA
BD-MED25 MED25 BD-MED25 F CGGAATTCATGTCGTCGGAGGTGAAACAGCTGATCGT
BD-MED25 R TTGGATCCcTCCCATGAAGCCAGCTCCAGGCATGTTA
AD-COI1 COI1 AD-COI1 F GGAATTCCATATGATGGAGGATCCTGATATCAAGA
AD-COI1 R TCCCCCCGGGTCATATTGGCTCCTTCAGGAC
AD-HAC1 HAC1 AD-HAC1 F CCGGCCATGGAGGCCATGAATGTTCAGGCTCACATGT
AD-HAC1 R TCCCCCGGGGGACCTGAGCCCCCAGCGACTTC
AD-HAC5 HAC5 AD-HAC5 F GGAATTCCATATGATGGCTCAGGGGCAGAATAGG
AD-HAC5 R CGCGGATCCGTCATTCAGGAGTGGAGGCCG
AD-HAC12 HAC12 AD-HAC12 F CCGGCCATGGAGGCCATGAATGTTCAGGCTCAC
AD-HAC12 R TCCCCCGGGGGTCAACCCGAGGTTCCAGCGAC
AD-HAC11-894 HAC1 AD-HAC11-894 F CCGGCCATGGAGGCCATGAATGTTCAGGCTCACATGT
AD-HAC11-894 R TCCCCCGGGGGTGTATGTTCAGTAGACTCTGCCA
AD-HAC1887-1,200 HAC1 AD-HAC1887-1,200 F CCGGCCATGGAGGCCCTGGCAGAGTCTACTGAACATACA
AD-HAC1887-1,200 R TCCCCCGGGGGATAAACTCGGCGTTGATTGGGA
AD-HAC11,200-1,698 HAC1 AD-HAC11,200-1,698 F CCGGCCATGGAGGCCCTCTCGTATTTGGATTCTG
AD-HAC11,200-1,698 R TCCCCCGGGGGACCTGAGCCCCCAGCGACTTC
AD-HAC11-710 HAC1 AD-HAC11-710 F CCGGCCATGGAGGCCATGAATGTTCAGGCTCACATGT
AD-HAC11-710 R TCCCCCGGGGGCTTCACAGGAATACAGACAGG
AD-HAC1260-710 HAC1 AD-HAC1260-710 F CCGGCCATGGAGGCCCCCATGATGGTACCTCAG
AD-HAC1260-710 R TCCCCCGGGGGCTTCACAGGAATACAGACAGG
AD-HAC1630-710 HAC1 AD-HAC1630-710 F CCGGCCATGGAGGCCGATCCGAGATTCAAAAATC
AD-HAC1630-710 R TCCCCCGGGGGCACAGGAATACAGACAGGGCAG
LCI MED25-nLUC MED25 MED25-nLUC F CGGGATCCATGTCGTCGGAGGTGAAACAGCTGATCGT
MED25-nLUC R ACGCGTCGACTCCCATGAAGCCAGCTCCAGGCATGTTA
MED25vWF-A-nLUC MED25 MED25-nLUC F CGGGATCCATGTCGTCGGAGGTGAAACAGCTGATCGT
MED25vWF-A-nLUC R ACGCGTCGACCTCCGAGATCAGGACAAGATAGA
MED25MD-nLUC MED25 MED25MD-nLUC F CGGGATCCATGAATTTTGTGGAGGCATGTGCTGCCT
MED25MD-nLUC R ACGCGTCGACGCCATCATCGGGGGCTATGCAG
MED25ACID-nLUC MED25 MED25ACID-nLUC F CGGGATCCATGACTTCACAATCCAAATATGTGAA
MED25ACID-nLUC R ACGCGTCGACATTTGGAATTTGTGGTTTAAACA
MED25GD-nLUC MED25 MED25GD-nLUC F CGGGATCCATGCAGCAACAGCAGCAGCAACAACAA
MED25-nLUC R ACGCGTCGACTCCCATGAAGCCAGCTCCAGGCATGTTA
cLUC-COI1 COI1 cLUC-COI1 F CGGGGTACCATGGAGGATCCTGATATCAAGA
cLUC-COI1 R ACGCGTCGACTCATATTGGCTCCTTCAGGAC
cLUC-COI1F-box COI1 cLUC-COI1 F CGGGGTACCATGGAGGATCCTGATATCAAGA
cLUC-COI1F-box R ACGCGTCGACCATAGTCACATGCTCTCTCGTCTC
cLUC-COI1LRR COI1 cLUC-COI1LRR F CGGGGTACCGCGCTTTGCTACACTGCGACGCC
cLUC-COI1 R ACGCGTCGACTCATATTGGCTCCTTCAGGAC
cLUC-HAC1 HAC1 cLUC-HAC1 F TCCCCCGGGATAATGTTCAGGCTCACATG
cLUC-HAC1 R ACGCGTCGACTTAACCTGAGCCCCCAGCGAC
Pull down pMAL-MED25551-836 MED25 pMAL-MED25551-836 F CGGGATCCATGACTTCACAATCCAAATATGTGAA
pMAL-MED25551-836 R GAAGTCGACTTAGTGGTGGTGGTGGTGGTGTCCCAT
GAAGCCAGCTCCAGGCATGTTA
pMAL-JAZ1 JAZ1 pMAL-JAZ1 F CGGAATTCATGTCGAGTTCTATGGAATGTTCTG
pMAL-JAZ1 R CGGGATCCGTGGTGGTGGTGGTGGTGTATTTCAGCT
GCTAAACCGAGC
pET28a-COI1 COI1 pET28a-COI1 F GGAATTCCATATGATGGAGGATCCTGATATCAAGA
pET28a-COI1 R ACGCGTCGACTCATATTGGCTCCTTCAGGAC
Antibody pET28a-HAC1 HAC1 pET28a-HAC1 F GCGTCGACGTAATGTTCAGGCTCACATGTCG
pET28a-HAC1 R ATAAGAATGCGGCCGCTACCTGAGCCCCCAGCGAC
pMAL-MYC2 MYC2 pMAL-MYC2 F GCTAGCATGACTGATTACCGGCTACAAC
pMAL-MYC2 R CTGCAGACCGATTTTTGAAATCAAACTTGC
Transformation pHAC1:HAC1-GFP HAC1 promoter HAC1 promoter F AAACTGCAGGTGAGTTTTGAGATCGATATG
HAC1 promoter R ACGCGTCGACAGCTACCTCTTCAGTCTC
HAC1 HAC1 CDS F ACGCGTCGACATGAATGTTCAGGCTCAC
HAC1 CDS R TCCCCCGGGGACCTGAGCCCCCAGCGAC
GFP GFP F GAAGGTACCATGGTGAGCAAGGGCGAGG
GFP R GAATTCCTTGTACAGCTCGTCCATGCCGA
p35S:JAZ1-GFP JAZ1 JAZ1 F CACCATGTCGAGTTCTATGGAATG
JAZ1 R TATTTCAGCTGCTAAACCGAGCC
p35S:MED25-Flag MED25 MED25 F CACCATGTCGTCGGAGGTGAAACAGCTGATCGT
MED25 R TCCCATGAAGCCAGCTCCAGGCATGTTA
p35S:MYC2-Flag MYC2 MYC2 F CACCATGACTGATTACCGGCTACA
MYC2 R ACCGATTTTTGAAATCAAACTTGC
ChIP JAZ8 JAZ8-A F GTTGTCGAAGTTTGTTGGTAGG
JAZ8-A R CTCGGTTGCAATTGTATCAAC
JAZ8-B F AAAACCTTAAAGTTGCACAAATG
JAZ8-B R TTTACAACGGCGGACTCTTAG
JAZ8-C F TGTCCTCTCTCTCCTCACAAATAG
JAZ8-C R ATAAGAAGTGGGAAAAAGACGAAG
JAZ8-D F GATCGCAAGCAGAGAAATGA
JAZ8-D R TATCGTCGTGAATGGTACGG
ERF1 ERF1-A F TATCGTATCAAAGGTCTCTCCCAA
ERF1-A R GCAACATTTTCTGTCGTTCGGA
ERF1-B F AAGAGATACAATGTCCAGGTTT
ERF1-B R ATTAGAACACTACACATCATCACA
ERF1-C F TCAATCCACTAACGATCCCTAA
ERF1-C R TTAGAAGAAGAGGATGAGAAAGAA
ERF1-D F GGCGGAGAGAGTTCAAGAGT
ERF1-D R TCCGTCTCATCGAGTGTTTC
ACTIN7 ACT7 F CCATAGCATTGTCTCTCCCA
ACT7 R CGGCAGCAGCTAACACTAAG
qRT-PCR VSP1 VSP1 F CCTCGAATCGAACACCATCT
VSP1 R GGCACCGTGTCGAAGTTTAT
PDF1.2 PDF1.2 F CGCACCGGCAATGGTGGAAG
PDF1.2 R CACACGATTTAGCACCAAAG
JAZ8 JAZ8 F TCGTCTTTTTCCCACTTCTTATGA
JAZ8 R AAGTCATCCTCAAACGGGTCG
ERF1 ERF1 F GGAAATTCGCGGCGGAGATAAGA
ERF1 R CAACGCCACAACCGGAGAACAAC
HAC1 HAC1 F CCCGATGAAGGAGGACTTTA
HAC1 R TCCCTGTCTTCACGTCTCTG
MED25 MED25 F GTCAAATGTCGCTTCAAACC
MED25 R CTGCCACCAAGTTGTTGTATT
ACTIN7 ACTIN7 F CCATTCAGGCCGTTCTTTC
ACTIN7 R CGTTCTGCGGTAGTGGTGA

ACID, activator-interacting domain; F, forward; GD, glutamine-rich domain; MD, middle domain; R, reverse.