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. 2017 Oct 2;114(42):11127–11132. doi: 10.1073/pnas.1711389114

Fig. 4.

Fig. 4.

Assessment of IAPP interaction with healthy pancreatic EVs by SEC and AFM. (A) SEC of freshly eluted IAPP (monomers). (B) SEC of EVs (elutes in the void), with AFM imaging of the indicated fraction. (C) SEC of a mixture of IAPP and EVs without incubation (elutes as monomeric IAPP and EVs in the void, i.e., as a combination of the traces in A and B). (D) SEC of IAPP (which forms amyloids and is filtered away before SEC injection) and a mixture of IAPP and EVs preincubated for 20 min at 37 °C before SEC analysis. The eluate contains no monomeric IAPP; instead, absorption of EVs in void is decreased, which may be explained by the formation of larger particles that are partially filtered away before SEC. The arrow points to reduced absorption of EVs after incubation with IAPP. (E) AFM image of an incubated mixture of IAPP and EVs (larger particles than in the AFM image in B).