Fig. S5.

Generation of Retnlb^−/− mice. (A) CRISPR/Cas mutagenesis was used to introduce edits into the mouse Retnlb exon 2 sequence encoding the RELMβ signal peptide (the guide RNA-targeted sequence is underlined). A mutation was selected that introduced a premature stop codon into the RELMβ signal peptide, and mice harboring this mutation were bred to homozygosity. (B) RELMβ is not expressed in colons from Retnlb^−/− mice. Mice were orally infected with C. rodentium, and then colons were analyzed for RELMβ expression by Western blot. Each lane represents a different mouse. (C) Bacterial counts from C. rodentium-infected wild-type and Retnlb^−/− colons. Mice were orally infected for 7 d. Assays were performed in triplicate, and means ± SD are shown. Statistics were performed with Student’s t test; *P < 0.05; **P < 0.01.