Fig. S6.

Determination of bacterial 16S copy number in small intestine, colon, MLN, and spleen from wild-type and Retnlb^−/− mice. (A) Q-PCR determination of 16S rRNA gene copy number in the distal small intestinal (ileal) lumen, ileal tissue, MLN, and spleen of cohoused wild-type and Retnlb^−/− mice. (B–F) Q-PCR analysis of 16S gene copy numbers from specific bacterial groups. Bacteria were recovered from the ileal lumen (B), ileal tissue (C), MLN (D), spleen (E), and colon lumen (F), and analyzed using taxon-specific 16S rDNA primers. (G) Q-PCR quantification of specific subgroups of Proteobacteria in the colon lumen. Values for each bacterial group are expressed relative to the 16S rDNA levels in wild-type mice. Four mice per genotype were analyzed for each experiment, and Q-PCR assays were repeated in triplicate within each experiment. Means ± SD are plotted. Statistics were performed with Student’s t test; *P < 0.05; ns, not significant; nd, not detected.