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. 2017 Oct 2;114(42):11151–11156. doi: 10.1073/pnas.1711754114

Fig. 2.

Fig. 2.

Four-helix bundle interface between S and M subunits. (A) Ribbon diagram of the four-helix bundle structure consisting of the CR regions (light blue) from the S subunit and two C-helices (yellow and violet) from the M subunits. The key residues are shown in stick representation. (BD) Detailed interactions within the four-helix bundle interface as highlighted by the dashed boxes in A. The hydrophobic interactions are shown in space-filling presentation (B and D). The hydrogen bonds are shown as thick black dashed lines (C). Same color code as in A. (EG) SDS/PAGE results of the coexpression of the wild-type S subunit (no tag) and different M subunits (His tagged) as indicated for both Tte (E and G) and EcoKI (F) systems. The SDS/PAGE gels were stained with Coomassie blue.