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. 2017 Oct 2;114(42):E8847–E8854. doi: 10.1073/pnas.1713925114

Table S1.

List of primers used in this study

Constructs Sequences (5′ → 3′) (forward and reverse)
Citrine-SYP1a construct (left arm) GGGGTACCAGCTCTAGCGCGTTGAATTG
ACGCGGGCCCAGTGACTCACTCTCAAGCAA (Underline = Kpn1 site and Apa1 site)
Citrine-SYP1a construct (right arm) GGACTAGTTATGAACAACCTTCTTAGCAACTCG
TCCCCGCGGCAAGGGAACTCGCTATGCTT (Underline = Spe1 site and Sac2 site)
Citrine-MAP65d construct (left arm) GGGGTACCTGAACGATTGTCGGAGTTGC
CCCAAGCTTTGTGGCTTCTACAAGATCACC (Underline = Kpn1 site and Hind3 site)
Citrine-MAP65d construct (right arm) GGACTAGTTATGGTTCAGCAGCCTGTGAC
ATTTGCGGCCGCCCATACCGACCCACTTCGTA (Underline = Spe1 site and Not1 site)
TON1 KO construct (left arm) GGGGTACCGATTGAGCCCTCTGCCAAAC
CCATCGATGTAAAACGACTTCTTACAACCTTCA (Underline = Kpn1 site and Cla1 site)
TON1 KO construct (right arm) CGGGATCCAAATTTGAAATTTCTCACCTTACGT
ATTTGCGGCCGCGAGTTTCACGTCACCTAACCAG (Underline = BamH1 site and Not1 site)
Lifeact-GFP construct CACCATGGGTGTCGCAGATTTGAT
TTACTTGTACAGCTCGTC
mCherry/α-tubulin construct CACCATGGTGAGCAAGGGCGAGGAG
GCTCCTGCTCCTGCTCCGCTGTACAGCTCGTCCATGCCGC
mCherry/α-tubulin construct AAAGCGGCCGCGGGCCCCACCATGGTGAGC
TTTACTAGTACCTGCTCCTGCTCCTGCTCCTGCTCCTGCTCCGC (Underline = Not1 site and Spe1 site)
mCherry/α-tubulin construct GGTACTAGTATGAGAGAGATTATC
TATGGCGCGCCGGGCCCTCAGTAGTCGTCGTCCTCCG (Underline = Spe1 site and Asc1 site)