Citrine-SYP1a construct (left arm) |
GGGGTACCAGCTCTAGCGCGTTGAATTG |
ACGCGGGCCCAGTGACTCACTCTCAAGCAA (Underline = Kpn1 site and Apa1 site) |
Citrine-SYP1a construct (right arm) |
GGACTAGTTATGAACAACCTTCTTAGCAACTCG |
TCCCCGCGGCAAGGGAACTCGCTATGCTT (Underline = Spe1 site and Sac2 site) |
Citrine-MAP65d construct (left arm) |
GGGGTACCTGAACGATTGTCGGAGTTGC |
CCCAAGCTTTGTGGCTTCTACAAGATCACC (Underline = Kpn1 site and Hind3 site) |
Citrine-MAP65d construct (right arm) |
GGACTAGTTATGGTTCAGCAGCCTGTGAC |
ATTTGCGGCCGCCCATACCGACCCACTTCGTA (Underline = Spe1 site and Not1 site) |
TON1 KO construct (left arm) |
GGGGTACCGATTGAGCCCTCTGCCAAAC |
CCATCGATGTAAAACGACTTCTTACAACCTTCA (Underline = Kpn1 site and Cla1 site) |
TON1 KO construct (right arm) |
CGGGATCCAAATTTGAAATTTCTCACCTTACGT |
ATTTGCGGCCGCGAGTTTCACGTCACCTAACCAG (Underline = BamH1 site and Not1 site) |
Lifeact-GFP construct |
CACCATGGGTGTCGCAGATTTGAT |
TTACTTGTACAGCTCGTC |
mCherry/α-tubulin construct |
CACCATGGTGAGCAAGGGCGAGGAG |
GCTCCTGCTCCTGCTCCGCTGTACAGCTCGTCCATGCCGC |
mCherry/α-tubulin construct |
AAAGCGGCCGCGGGCCCCACCATGGTGAGC |
TTTACTAGTACCTGCTCCTGCTCCTGCTCCTGCTCCTGCTCCGC (Underline = Not1 site and Spe1 site) |
mCherry/α-tubulin construct |
GGTACTAGTATGAGAGAGATTATC |
TATGGCGCGCCGGGCCCTCAGTAGTCGTCGTCCTCCG (Underline = Spe1 site and Asc1 site) |