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. 2017 Oct 20;7:13658. doi: 10.1038/s41598-017-13287-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Concordance between Roche TaqMan and GeneXpert assays. Percentages of samples reported ‘detected’ or ‘not detected’ using the qualitative Xpert HIV-1 Qual (a,b) and quantitative HIV-1 Viral Load cartridges (c,d), with and without incorporating a 3 minute centrifugation step from a 100 µl whole blood finger-prick sample. Panels a. and c. show participants with undetectable plasma viraemia using the Roche TaqMan assay tested using whole blood finger-prick samples using the GeneXpert Qual and Viral Load cartridges, respectively. Panels b and d show participants with detectable viraemia using the Roche TaqMan assay tested using the Qual and Viral Load cartridges, respectively. Arrows indicate the impact of introducing a 3 minute centrifugation ‘spin’ step to the protocol.