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. 2017 Oct 20;7:13658. doi: 10.1038/s41598-017-13287-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Impact of benchtop centrifugation on PoC finger-prick viral load quantification. Comparison of Roche TaqMan pVL assay with GeneXpert Viral Load assay without (a) or with (b) a 3 minute centrifugation step. Vertical line in (a) shows suggested lower limit of detection for the finger-prick protocol (1000 copies/ml) based on this study. Correlation between Roche TaqMan pVL assay and Xpert Viral Load assays when a lower limit of detection of 1000 copies/ml was imposed and 6 samples with pVL below this excluded (d). Values are Spearman’s Rho and P values. Bland Altman analysis comparing the Roche TaqMan with Xpert VL assay with centrifugation for samples > 1000 copies/ml (d).