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. 2017 Oct 20;7:13675. doi: 10.1038/s41598-017-14223-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Antibiotic treatment decreased IL-17-producing cells in the spleen and kidney and increased IL-10 in the circulation. (A) Serum level of IL-6 at 16 weeks of age (n ≥ 7 per group). (B) Intracellular staining of IL-17 and the percentage of IL-17-producing cells in the spleen at 16 weeks of age (n = 4 per group). (C) FACS analysis of CD4⁺RORγ⁺ Th17 cells and CD4⁻RORγ⁺ ILC3 cells in the spleen at 16 weeks of age (n = 4 per group). (D,E) FACS analysis of DN-T cells (D) and Th17 cells (E) in the kidney at 16 weeks of age (n = 4 per group). (F) Protein level of IL-17 in the spleen at 16 weeks of age (n ≥ 7 per group). (G) Serum level of IL-10 in the mouse serum at 16 weeks of age (n ≥ 7 per group). *p < 0.05, **p < 0.01.