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. 2017 Oct 23;474(21):3615–3626. doi: 10.1042/BCJ20170588

Figure 4. The RING domains of VPS41 and VPS18 are required for efficient interaction with endogenous HOPS components.

Figure 4.

HEK293T cells were transfected with GFP-tagged VPS41 and VPS18 truncation constructs. After immunoprecipitation (IP), samples were immunoblotted for the indicated endogenous HOPS subunits (VPS11, VPS18, VPS33A, and/or VPS41), GAPDH (loading control), and GFP. (A) Schematic showing truncation constructs. (B) The RING domain of VPS41 (778–854) integrates into the endogenous HOPS complex. (C) While the RING domain of VPS18 (842–973) is able to interact with endogenous VPS33A and VPS41, an extended construct (752–973) is required to co-immunoprecipitate VPS41 as efficiently as full-length (1–973) VPS18.