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. 2017 Oct 20;7:13694. doi: 10.1038/s41598-017-14123-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genome-scale analysis of 5′UTR and functional prediction of regulatory ncRNAs. (a) Distribution of 1,223 5′UTRs of E. limosum, with a major peak at length 20–29 nt. (b) The conserved ribosome binding site (RBS) motif (GGAGR) for protein coding genes in the 5′UTRs is longer than 8 nt. The bottom panel shows conservation of each motif of RBS. (c) Classification of ncRNAs using antisense, intergenic, and primary TSSs, according to their locations. The functions of the classified RNAs were predicted using the Rfam database. (d) Moco-RNA motif containing ncRNA, which was identified by dRNA-Seq, was located upstream of the hydrogenase complex at genome position 3,421,126, which was confirmed via the Rfam database.