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. 2017 Oct 20;7:13712. doi: 10.1038/s41598-017-14146-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Homodimer formation of TH1 protein detected via yeast two-hybrid assay. The WT and three mutant alleles of the TH1 gene were cloned into both the bait vector pGBKT7 and the prey vector pGADT7 and co-transformed into the yeast strain Y187. A decimal serial dilution of the yeast colony was spotted onto the selective medium DDO (SD/-Trp-Leu) or QDO (SD/-Trp-Leu-Ade-His). The colony will survive on the QDO medium only if the bait and prey interact with each other. pGADT7-RecT and pGBKT7-53 was used as positive control, while pGADT7-RecT and pGBKT7-Lam as negative control.