Figure 8.

Requirement of the TH1 transcriptional repression activity in spikelet development. (a) Schematic representation of the vectors transformed into the s2-89 mutant. Four copies of transcription activation domain VP16 or repression domain EAR were fused with the TH1^s2-89 mutant protein. (b) Spikelet morphology of transgenic lines expressing the VP16-TH1^s2-89 fusion protein (B192-2) and the EAR-TH1^s2-89 fusion protein (B193-2) in s2-89 mutant. (c) Comparisons of grain length, grain width, length-width ratio and grain thickness in WT, s2-89 and the transgenic lines. Data are means ± SE (n = 20). Double asterisks indicate significant differences at P < 0.01 by Student’s t test. Each scale bar is indicated.