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. 2017 Oct 20;7:13713. doi: 10.1038/s41598-017-14175-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Partial purification and enrichment of aSyn from erythrocyte lysates for PTM analyses. (a) Schematic of aSyn enrichment. Erythrocyte crude lysates (E) are first thermo-enriched (TE) and then haemoglobin-depleted (TE-HD). (b) Coomassie staining of an SDS-PAGE separation of 5 μg of E, TE and TE-HD. Arrow indicates the MW of aSyn. (c) Western blot analysis of 5 μg of E, TE and TE-H probed with an antibody against aSyn. (d) Dot blot analysis of 5 μg of E, TE and TE-HD extracts probed with an antibody against aSyn. (e) Schematic of the aSyn protein sequence highlighting the PTMs analysed: Glycation (yellow), SUMOylation (green), nY39 (red) and pY125 (blue).