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. 2017 Oct 20;7:13713. doi: 10.1038/s41598-017-14175-5

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of the measurement of the levels of the PTMs in erythrocytes. After the enrichment procedure described in Fig. 1a, TE-HD extracts are applied onto a nitrocellulose membrane using a dot blot system. The membranes are probed for the PTMs of interest using specific antibodies and, after stripping, for total aSyn. The ratio between the PTMs and the levels of aSyn is determined. The average corresponding to the control group is then used to normalize all levels. Data is presented as PTM/aSyn fold of control.