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. 2017 Oct 20;7:13654. doi: 10.1038/s41598-017-13233-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Kinetic properties of GluA2Q/2R complex channels with different R/G editing status. (a) Representative whole-cell current traces (10 mM glutamate) from GluA2Q_flip/2R_flip (left) and GluA2Q_flop2R_flop (right) channels with four different R/G editing combinations, labeled in different colors and letters. For example, GR refers to the channel that contains the R/G edited in the GluA2Q and R/G unedited in the GluA2R isoforms (these are the same labels in Table 2). (b) Current-voltage relationships for GluA2Q_flip/2R_flip and GluA2Q_flop/2R_flop with different R/G editing status. Peak current amplitudes were measured from HEK-293 cells voltage clamped from −100 mV to +60 mV), and were normalized to current amplitudes at −60 mV. (c) Dependence of desensitization rate (k_des) on glutamate concentration for via GluA2Q_flip/2R_flip (left) and GluA2Q_flop/2R_flop (right) channels. (d) The k_op values of GluA2Q_flip/2R_flip (left) and GluA2Q_flop/2R_flop (right) channels, full pairwise comparisons were performed for flip and flop, respectively. (One-way ANOVA with Tukey’s correction; flip: p = 0.77, F_3,5 = 3.96; flop(RR) versus flop(RG), p ≤ 0.05, n = 3; flop(RR) versus flop(GR), p ≤ 0.05, n = 3; flop(RR) versus flop(GG), p = 0.98, n = 3; flop(RG) versus flop(GR), p ≤ 0.05, n = 3; flop(GG) versus flop(RG), p ≤ 0.05, n = 3; flop(GG) versus flop(GR), p ≤ 0.05, n = 3). (e) The k_cl values of GluA2Q_flip/2R_flip (left) and GluA2Q_flop/2R_flop (right) channels, full pairwise comparisons were performed for flip and flop, respectively. (One-way ANOVA with Tukey’s correction; flip: p = 0.73, F_3,5 = 4.33; flop(RR) versus flop(RG), p = 0.60, n = 3; flop(RR) versus flop(GR), p ≤ 0.05, n = 3; flop(GG) versus flop(RG), p ≤ 0.05, n = 3; flop(GG) versus flop(GR), p = 0.35, n = 3;flop(GG) versus flop(RR), p ≤ 0.05, n = 3; flop(GR) versus flop(RG), p ≤ 0.05, n = 3). (f) K₁ values of GluA2Q_flip/2R_flip (left) and GluA2Q_flop/2R_flop (right) channels. All the K₁ values are determined from non-linear fitting of the dose-response data to equation, fittings are shown in Supplementary Fig. S1b,c. (3) (One-way ANOVA with Tukey’s correction; flip, p = 0.60, F_3,5 = 4.47; flop, p = 1.01, F_3,5 = 4.88). The detailed linear fittings in both (d) and (e) are provided in Supplementary Figs. S2 and S3. All of the constants are summarized in Table 2. *p ≤ 0.05.