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. 2017 Oct 20;7:13654. doi: 10.1038/s41598-017-13233-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the R/G editing in GluA2R on the kinetic properties of GluA1/2R heteromeric channels. (a) Representative whole-cell current responses of GluA1_flop/2R(R)_flop (left) and GluA1_flop/2R(G)_flop (right) channels to 10 mM glutamate. (b) Maximum k_des values (at saturated glutamate concentrations) of GluA1/2R with different flip/flop and R/G editing (in GluA2R) status. From left to right, GluA2R in the flip form is shown in columns 1–4 and the flop form is in columns 5–8. All the columns are also shown in specific patterns, and the bar codes are shown in the box on top of panel (b). One-way ANOVA with Tukey’s correction was done for columns 1–4 or group 1(flip group) and columns 5–8 or group 2 (flop group), respectively (n = 3). Each group was subjected to full pairwise comparisons; yet, for clarity, only those pairs that were statistically significant between R and G are labeled in the figure (Group 1: columns 1 and 2, p ≤ 0.05; columns 1 and 3, p = 0.75; columns 1 and 4, p = 0.54; columns 2 and 3, p ≤ 0.05; columns 2 and 4, p ≤ 0.05; columns 3 and 4, p = 0.36. Group 2: columns 5 and 6, p ≤ 0.05; columns 5 and 7, p ≤ 0.05; columns 5 and 8, p ≤ 0.05; columns 6 and 7, p ≤ 0.05; columns 6 and 8, p ≤ 0.05; columns 7 and 8, p ≤ 0.05). (c) A pair of representative whole-cell current traces from the opening of GluA1_flop/2R(R)_flop and GluA1_flop/2R(G)_flop channels initiated by a laser-pulse photolysis of caged glutamate at time zero. The observed channel opening rate constant, k_obs, was determined by fitting the rising phase to a single-exponential rate expression (equation (1)), shown as the solid line. (d) Linear fit of k_obs as a function of glutamate concentration (by equation (2)) of GluA1_flop/2R(R)_flop (R, Δ) and GluA1_flop/2R(G)_flop channels (G, ○), respectively. The k_op and k_cl values are shown in Tables 3 and 4. (e) The k_op values of various GluA1/2R channels with different R/G editing and flip/flop status. From left to right, GluA2R in the flip form is shown in columns 1–4 while the flop form is in columns 5–8. One-way ANOVA with Tukey’s correction was done for columns 1–4 or group 1(flip group) and columns 5–8 or group 2 (flop group), respectively (n = 3). Each group was subjected to full pairwise comparisons; yet, for clarity, only those pairs that were statistically significant between R and G are labeled in the figure (Group 1: columns 1 and 2, p = 0.88; columns 1 and 3, p = 0.85; columns 1 and 4, p ≤ 0.05; columns 2 and 3, p = 0.78; columns 2 and 4, p ≤ 0.05; columns 3 and 4, p ≤ 0.05. Group 2: columns 5 and 6, p = 0.11; columns 5 and 7, p ≤ 0.05; columns 5 and 8, p ≤ 0.05; columns 6 and 7, p ≤ 0.05; columns 6 and 8, p ≤ 0.05; columns 7 and 8, p ≤ 0.05). The detailed linear fittings in (e) are provided in Supplementary Fig. S6. All the constants are summarized in Tables 3 and 4. *p ≤ 0.05.