Figure 2.

Ubiquitous Panx1 deletion does not affect atherogenesis. Sudan-IV staining (A) and quantification of atherosclerotic lesion extent in the thoracic-abdominal aortas (B) and in the aortic roots (C) of Apoe ^−/− (white bars) and Panx1 ^−/− Apoe ^−/− (grey bars) mice after 5 weeks of HCD (n = 10). Scale bar represents 200 μm. (D) Representative image illustrating the difference in size between Apoe ^−/− and Panx1 ^−/− Apoe ^−/− mice at the age of 10 weeks (n = 10). (E) Weights of Apoe ^−/− and Panx1 ^−/− Apoe ^−/− mice before and after 5 weeks of HCD (n = 10). Fat mass (F) and lean mass (G) of Apoe ^−/− and Panx1 ^−/− Apoe ^−/− mice were measured by MRI (n = 5). Subcutaneous adipose tissue (SAT; H) and visceral adipose tissue (VAT; I) in Apoe ^−/− and Panx1 ^−/− Apoe ^−/− mice was determined by micro X-ray computed tomography (n = 10). Serum total cholesterol (J), TG (K), FFA (L), LDL (M) and HDL (N) levels in Apoe ^−/− and Panx1 ^−/− Apoe ^−/− mice were measured after 5 weeks of HCD (n = 10).