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. 2017 Oct 20;7:13682. doi: 10.1038/s41598-017-10946-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Titrations of RK-10-Cy5 in Cell Lines using flow cytometry. Cell lines expressing high PD-L1 (MDA-MB-231) and low PD-L1 (MCF7, Y79) were incubated with fluorescent RKC-10-Cy5 peptide and anti-cytokeratin for 1 hour at shown concentrations, then analyzed using flow cytometry. In some cases we can distinguish populations of PD-L1 expression, separated by color. Higher concentrations of peptide were unable to distinguish expression, while concentrations of 0.005 and 0.001 mg/mL peptide were selected as ideal for flow cytometry.