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. 2017 Oct 20;7:13682. doi: 10.1038/s41598-017-10946-2

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of PD-L1 expression in patient tissues using RK-10-Cy5. RKC-10-Cy5 peptide was incubated with patient melanoma and squamous cell carcinoma samples for 1 hour according to clinical protocols. Patient tissues were compared to MDA-MB-231 (PD-L1 high positive) and normal blood (PD-L1 negative) in order to asses PD-L1 expression. The SCC sample showed very high PD-L1 expression, while the melanoma sample showed a moderate expression of PD-L1.