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. 2017 Oct 20;7:13661. doi: 10.1038/s41598-017-13890-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Secreted embryonic alkaline phosphatase (SEAP) release from untreated, mock-transfected, or MSR1-transfected HEK-Blue hTLR9 cells using HEK-Blue detection solution. Each DNA sample was added to the cells at a final concentration of 50 μg/mL, and the OD of the sample was measured at 620 nm. All oligodeoxynucleotides (ODNs) have a phosphodiester backbone except for CpG2006. The results are expressed as means + SEM of three independent experiments. *P < 0.05 compared with the mock-transfected group.