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. 2017 Oct 20;7:13708. doi: 10.1038/s41598-017-14053-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Light microscopic images of whole-mount preparations (a,b) and a tissue section (c) of vascularized iPSC-CM 3D-tissues 4 days after construction. (a,c) Immunostaining for sarcomeric α-actinin (red) and CD31 (green) shows a meshwork of iPSC-CMs preparing myofibrils and a network of endothelial tubes with lumens. (b) Immunostaining for cTnT (red) and Cx43 (green) shows sarcomeres in iPSC-CMs, and a small amount of reaction products for Cx43 on the cells. Cellular nuclei are stained with DAPI. (d) An image of reaction products for Cx43 distilled from b.