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. 2017 Oct 20;7:13708. doi: 10.1038/s41598-017-14053-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TEM images of vascularized iPSC-CM 3D-tissues 4 days after construction. (a) The iPSC-CMs (CM) are assembled to contact with each other within some amount of extracellular matrices (ECM) and the endothelial (blue) tubes (ET) extends along the iPSC-CMs. FB, fibroblast. (b) Higher magnification of the boxed area in a. Numerous myofilaments assemble to form myofibrils (mf), whereas some myofilaments with poor connectivity (white arrow) are also seen. Small mitochondria (m) with a few cristae are sparsely distributed within sarcoplasm. Black arrows, white and black arrowheads indicate sarcoplasmic reticula, Z-bands and desmosomes between the adjacent iPSC-CMs, respectively. (c) Higher magnification of the contact area of the iPSC-CMs. Many desmosomes (black arrowheads) are seen between the cells, but gap junction is indistinguishable. The cell at the top shows intracellular edema. White arrowheads indicate Z-bands of myofibrils (mf). m, mitochondria. (d) Higher magnification of the boxed area in a. The endothelial cells connect to each other by overlapping (arrows) with junctional complex (black arrowheads) to form vascular lumen. The endothelial pores (white arrowheads) are also seen. (e) A insertion of extracellular tissue (*) is seen in the endothelial cords (EC). CM, iPSC-CM. Inset: Higher magnification of the boxed area in e. The endothelial cell contains abundant rough endoplasmic reticula (rer), coated vesicles (black arrows), Golgi apparatuses (G) and lysosomes (white arrow). Scale bar: 500 nm.