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. 2017 Oct 20;7:13708. doi: 10.1038/s41598-017-14053-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of vascular distribution in iPSC-CM 3D-tissues 28 days after implantation to rat infarcted heart. (a) The ratio of the CD31-immunopositive vascular area to unit area in the deep region (0.76 ± 0.19%; n = 3, total 6 fields) is lower than that in the intermediate (1.03 ± 0.31%; n = 3, total 6 fields) or superficial (1.04 ± 0.52%; n = 3, total 6 fields) region within the implant, though there is no statistical difference among each region. (b) A representative image of three divided regions in the implant and vascular distribution in each region. Immunostaining for cTnT (red) and multi-species CD31 (green) on a transverse section of the iPSC-CM 3D-tissue implanted to the infarcted rat heart. The pericardium is located at the top of the image.