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. 2017 Oct 20;7:13640. doi: 10.1038/s41598-017-14162-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chronic mild stress (MS) for 3 weeks increased the number of mast cells. Toluidine blue staining detected mast cell numbers in the white adipose tissue (WAT) (a), volar skin of the lower legs (b), and brain (c) from control (CNT) and MS mice. Scale bar, 50 μm (a and b) and 100 μm (c). Blue bars indicate CNT mice, and red bars indicate MS mice. All data are expressed as the means ± SEM (a,b: n = 6/group, c: n = 13/group). *p < 0.05, versus CNT. Abbreviations: field CA3 hippocampus (CA3).