Fig. 2.

Muscular dystrophy amelioration in absence of Nfix persists up to 6 months. a Hematoxylin and eosin (H&E) and Milligan’s trichrome staining of tibialis anterior (left) and diaphragm (right) muscles at 8 weeks of age; N = 23 Sgca null and 12 Sgca null:Nfix null mice. Scale bar 100 μm. b Hematoxylin and eosin (H&E) and Milligan’s trichrome staining of tibialis anterior (left) and diaphragm (right) muscles at 12 weeks of age; N = 6 Sgca null and 4 Sgca null:Nfix null mice. Scale bar 100 μm. c Hematoxylin and eosin (H&E) and Milligan’s trichrome staining of tibialis anterior (left) and diaphragm (right) muscles at 6 months of age; N = 4 Sgca null and 4 Sgca null:Nfix null mice. Scale bar 100 μm. See also Supplementary Fig. 1 for the analysis of central nucleation in tibialis anterior and diaphragm muscles at 8 and 12 weeks and for the analysis of CSA at 8 weeks, Supplementary Fig. 2 for full muscle reconstructions, collagen I quantification, histology of gastrocnemius, quadriceps, soleus, and EDL muscles, and PCR analysis of oxidative fiber genes at 8 weeks, and Supplementary Fig. 3 for the analysis of utrophin and myostatin levels at 8 weeks