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. 2017 Oct 20;8:1055. doi: 10.1038/s41467-017-01098-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Nfix overexpression in skeletal muscle exacerbates the dystrophic phenotype. a Hematoxylin and eosin (H&E) and Milligan’s trichrome staining of tibialis anterior (left) and diaphragm (right) muscles at 5 weeks of age; N = 4 Sgca null and 9 Sgca null:Mlc1f-Nfix2 mice. Scale bar 100 μm. b Hematoxylin and eosin (H&E) and Milligan’s trichrome staining of tibialis anterior (left) and diaphragm (right) muscles at 8 weeks of age; N = 6 Sgca null and 30 Sgca null:Mlc1f-Nfix2 mice. Scale bar 100 μm. c Percentage of centrally nucleated myofibers in tibialis anterior muscles at 8 weeks of age; N = 11 Sgca null and 18 Sgca null:Mlc1f-Nfix2 mice. Mean ± SD; t test, ***P < 0.001. d Myofiber cross-sectional area distribution at 8 weeks of age; N = 186 fibers for WT, 908 for Sgca null, and 1026 for Sgca null:Mlc1f-Nfix2 mice. Mean ± whiskers from min to max; one-way ANOVA with Bonferroni post-test, ***P < 0.001. See also Supplementary Fig. 4 for PCR and WB analysis of Nfix expression in Sgca null:Mlc1f-Nfix2 mice, as well as for central nucleation and CSA measurements