Fig. 6.

Targeting Nfix ameliorates muscular dystrophy also in mdx mice. a Hematoxylin and eosin (H&E) and Milligan’s trichrome staining of tibialis anterior (left) and diaphragm (right) muscles at 10 weeks of age; N = 5 mdx and 4 mdx:Nfix null mice. Scale bar 100 μm. b Percentage of centrally nucleated myofibers in tibialis anterior (TA) and diaphragm muscles at 10 weeks of age; N = 5 mdx and 4 mdx:Nfix null mice for TA. N = 8 mdx and 5 mdx:Nfix null mice for diaphragm. Mean ± SD, t test, *P < 0.05; ***P < 0.001. See also Supplementary Fig. 3 for the analysis of utrophin and myostatin levels in mdx mice