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. 2017 Oct 20;7:13705. doi: 10.1038/s41598-017-13789-z

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Albumin colocalizes with megalin and cubilin in human podocytes. Immunolabeling shows colocalization (in yellow/orange) of megalin (left) and cubilin (right) with albumin after 24 hours of incubation. Lower panel are zoom-in of respectively upper images. Statistical analysis using Pearson’s coefficient (Rr) with LAS-AF software disclosed a significant correlation between cubilin and FITC-BSA (Rr = 0.71), and a non-significant correlation between megalin and FITC-BSA (Rr = 0.45). Green: FITC-BSA; Red: megalin (left) or cubilin (right); White arrows indicate colocalization. Blue: DAPI. Objective 20X/0.4. Scale bar 50 µm.