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. 2017 Oct 20;8:1079. doi: 10.1038/s41467-017-01052-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Chemical inhibition of CREB3L1 blocks metastasis. a A western blot of CREB3L1 expression in full-length CREB3L1 or CREB3L1^Δ375–519 transduced SUM159 cells that were treated with solvent control or a S1P inhibitor, AEBSF. b SUM159 cells transduced with control or CREB3L1^Δ375–519 were treated with solvent control or AEBSF. qPCR analyses were performed to quantify the expression of ECM genes: COL1A1 and COL1A2. The gene expression was normalized to the solvent-treated control transduction samples, n = 3. c Western blot of phosphorylated FAK (pY397 and pY576), total FAK, and Tubulin in cells of b. d Quantification of cell invasion of cells from b in a basement membrane-coated transwell assay, n = 3. e Two weeks after orthotopical transplantation with control or CREB3L1^Δ375–519 transduced MDA.MB.231-Luc-LM2 cells, NOD/SCID mice were treated with solvent control or AEBSF for another 4 weeks. Lung metastases of the animals were gauged by radiance from the whole lung in each animal, n = 9. Data are represented as mean ± SEM or geometric mean alone. * indicates p < 0.05; ** indicates p < 0.01 (Student’s t-test)