. 2017 Oct 20;7:13716. doi: 10.1038/s41598-017-14091-8

Table 5.

Details of PCR primers and conditions for genotyping of study SNPs.

Gene	rsID (location)	Primer (5′-3′)	Annealing temperature (°C)	Amplicon size (bp)	Restriction Enzyme	Allele size (bp)
CCL2	rs1024611 (−2518A/G)	F-TCTCTCACGCCAGCACTGACC	56	238	PvuII	G = 159,75 A = 234
	rs1024611 (−2518A/G)	R-GAGTGTTCACATAGGCTTCTG	56	238	PvuII	G = 159,75 A = 234
	rs2857656 (−362G/C)	F-GAGCCTGACATGCTTTCATCTA	58	174	Hpy188I	G = 113,61 C = 174
	rs2857656 (−362G/C)	R-TTTCCATTCACTGCTGAGAC	58	174	Hpy188I	G = 113,61 C = 174
CCL5	rs2280788 (−28C/G)	F-ACTCCCCTTAGGGGATGCCCG	55	175	HincII	C = 152,23 G = 175
	rs2280788 (−28C/G)	R-GCGCAGAGGGCAGTAGCAAT	55	175	HincII	C = 152,23 G = 175
	rs2107538 (−403G/A)	F-CACAAGAGGACTCATTCCAACTCA	50	206	RsaI	G = 180,26 A = 206
	rs2107538 (−403G/A)	R-GTTCCTGCTTATTCATTACAGATCGTA	50	206	RsaI	G = 180,26 A = 206
CCR2	rs1799864 (V64I)	F-TTGGTTTTGTGGGCAACATGATGG	56	173	BsaBI	A = 149,24 G = 173
CCR2	rs1799864 (V64I)	R-CATTGCATTCCCAAAGACCCACTC	56	173	BsaBI	A = 149,24 G = 173
CCR5	rs1799987 (59029 G/A)	F-CCCGTGAGCCCATAGTTAAAACTC	65	258	Bsp1286I	G = 130 A = 258
CCR5	rs1799987 (59029 G/A)	R-TCACAGGGCTTTTCAACAGTAAGG	65	258	Bsp1286I	G = 130 A = 258

Abbreviations: rs ID, reference SNP cluster ID; bp, base pair.