Fig. 7.

PTP4A1 inhibits the basal autophosphorylation of SRC. a Mean ± SEM of relative ratio of firefly/Renilla luciferase signal of HEK 293T cells co-transfected with human HA-tagged PTP4A1 WT or PTP4A1 C104S (phosphatase dead)-encoding pCDNA4 vector together with a firefly luciferase SMAD reporter and a control Renilla luciferase vector. Cells were stimulated with TGFβ1. Graph is representative of three independent experiments. b–d Mean ± SEM of SMAD3 or SRC mRNA expression or densitometric scan relative expression plus representative immunoblotting for SMAD3, SRC, pERK1/2 (T202/Y204) (upper bands) normalized to GAPDH or αtubulin (lower bands) in three different NHDF lines treated with control ASO or PTP4A1 ASO3. e Mean ± SEM of densitometric scan expression plus representative immunoblotting for pSRC (Y416) (upper bands) normalized to SRC (lower bands) in three different in vitro SRC kinase assay followed by incubation with PTP4A1/PTP4A2. f Densitometric scan expression plus representative immunoblotting for PTP4A1/2 (upper bands) normalized to SRC (lower bands) in two different in vitro SRC pull-down assay in presence of PTP4A1 or PTP4A2. g Mean ± SEM of densitometric scan expression plus representative immunoblotting for pSRC (Y416) (upper bands) normalized to SRC (lower bands) in three different in vitro SRC kinase assay carried out in presence of PTP4A1/PTP4A2. Paired t-test