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. 2017 Oct 20;7:13707. doi: 10.1038/s41598-017-14160-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FtsZ-GDP binding as a function of receptor density. Nonspecific adsorption (a) and specific binding (b–e) of FtsZ-GDP plotted as a function of the concentration of free FtsZ-GDP for various densities of ZipA, proportional to the percentage of NTA-bearing lipid coating the beads: (a) 0% NTA, (b) 0.5% NTA, (c) 2% NTA, (d) 5% NTA, (e) 10% NTA. Symbols are the data. Horizontal dotted lines are the amount of FtsZ that would completely cover the bead as a monolayer, calculated as described in the text. Curves were calculated using equations (1)–(3) with the following parameter values: A ₁ = 0; A ₂ = 0.00363; c ₅₀ = 150; $c_{s p e c}^{(m a x)} = 37$ (b), 38 (c), 58 (d), and 116 (e).