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. 2017 Oct 20;7:13692. doi: 10.1038/s41598-017-14136-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Single cell analysis and functional experiments in the wing disc. (A) Schematic drawing illustrates that cells in the center of the pouch are small, round and supposed to be mechanically compressed, whereas marginal cells are larger, elongated and mechanically stretched. In the marginal cells, the short edges are exposed to more mechanical stress than the long edges. These assumptions lead to following comparisons: (A’) The FRET index did not correlate with cell area in the wing pouch (here shown for shgFRET, R represents the Pearson correlation coefficient). (A”) The FRET index did not differ between long and short edges for shgContr (0.228 vs. 0,227) and shgFRET (0.240 vs. 0.240). (A”’) The FRET index did not differ between round and elongated cells for shgContr (0.229 vs. 0.226) and shgFRET (0.240 vs. 0.240). (Data for A’, A” and A”’ were pooled from 14 wing discs; n > 17.000 cells, n > 40.000 edges). (B) LatrunculinB treatment reduces cortical tension. (B’) FRET index decreased for shgContr (2.7%, n = 18) and shgFRET(0.4%, n = 18) upon treatment within 5 minutes. (detailed figure in Fig. S2C) (C) Using a stretching device, a pre-stretched (applied force of 10µN) and a stretched (25 µN) wind disc were compared. The dashed rectangle indicates the analyzed area. (C’) When cyclically altered between the two states every 5 minutes, a strong decay over time was observed for shgContr (18%, n = 2) and shgFRET (23%, n = 2), but no impact of the force change.