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. 2017 Oct 20;7:13692. doi: 10.1038/s41598-017-14136-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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FRET analysis of border cell migration. (A) During stage 9 in the egg chamber, a cluster of six to eight border cells (red) and two polar cells (blue) migrate from the anterior pole through the nurse cells towards the oocyte (path as dashed line). (B) Border cell cluster forms protrusions at the leading cell to navigate between the nurse cells. FRET index was detectable in the entire cluster, here illustrated in the YFP channel and the corresponding FRET index. (C) The relative front to back ratio of the FRET index was significantly lower for shgContr (0.95, n = 40) than for shgFRET (1.01, n = 38). (C’) But the absolute values between front and back did not differ significantly for shgContr (0.294 vs. 0.310, n = 40) nor for shgFRET (0.313 vs. 0.311, n = 38). (D) The release of tension upon the treatment with the myosin blocker Y-27632 did not significantly change the FRET index for shgContr (0.312 vs. 0.317, n = 40) nor for shgFRET (0.320 vs. 0.313, n = 38).