Table 2.
Comparison of thermostable fungal laccases.
| Organism | Enzyme | Res 1 | Res 2 | Res 3 | Topt, [°C] | T50, [°C] | Substratea | Reference | |
|---|---|---|---|---|---|---|---|---|---|
| Ascomycetes | Botrytis aclada | WT | V | T | S | nr | 69 | ABTS | this study |
| Botrytis aclada | T383I | V | I | S | 75 | ABTS | this study | ||
| Melanocarpus albomyces (VTT D-96490) | V | I | A | 60–70 | 60 | ABTS, GUA | 49,50 | ||
| Myceliophthora thermophila (CBS117.65) | I | V | A | nr | 70 | SGZ | 51,52 | ||
| Basidiomycetes | Ceriporiopsis subvermispora | L1 | V | T | P | nr | 60 | ABTS | 53 |
| Pleurotus ostreatus | POXC | V | L | P | 50–60 | 60 | ABTS | 54 | |
| Pycnoporus cinnabarinus (CBS 101046) | V | L | P | nr | 80 | SGZ | 55 | ||
| Pycnoporus sanguineus (CCT-4518) | V | L | P | 50 | 80 | SGZ | 56 | ||
| Steccherinum ochraceum (1833) | Laccase III | I | L | P | 80 | 70 | ABTS | 57 | |
| Trametes sp. (AH28–2) | LacA | V | L | P | 50 | 75 | GUA | 58 | |
| Ganoderma lucidum (KMK2) | I | L | P | 60 | 60 | ABTS | 59 | ||
| Basidiomycete PM1 (CECT 2971) | V | L | P | 80 | nr | ABTS | 60 |
Res 1, Res 2 and Res 3 indicate the amino acids found in these laccases in the corresponding positions of V105, T383 and S484 of Botrytis aclada laccase; Topt indicates the optimal temperature reported for the laccase assay, T50 indicates the temperature at which 50% residual activity is retained.
aABTS, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid; GUA, guaiacol; SGZ, syringaldazine.
nr, not reported.