FIG. 1.

Flow cytometric analysis of CLL-1 expression on AML blast and CD34⁺ leukemia cells in patient #70 and patient #58. (A) Patient #70, the bulk blast population in AML was characterized by low expression of CD45 and low side scatter (CD45^dim/SSC^low). Granulocytes were excluded based on SSC properties. The percentage of CLL-1 bulk blast is 85.11%. (B) The blast cells were back-gated into a forward scatter (FSC)/SSC plot to ensure homogeneous scatter property of the blast population. (C) Gate on blast population, IgG2B-APC isotope control was used as negative controls of CLL-1 expression in blast cells. (D) According to the gate of isotope controls in blast cells, percentage of CLL-1-positive leukemia cells are 57.75%, which is higher than 42.5% and classified into the CLL-1^high group. Patient #58 dot plot (E, F) as the same description as the patient #70. (G) Gate on blast population, IgG2B-APC isotope control was used as negative controls of CLL-1 expression in blast cells. (H) According to the gate of isotope controls in blast cells, percentage of CLL-1-positive leukemia cells are 27.48%, which is less than 42.5% and classified into the CLL-1^low group. AML, acute myeloid leukemia; CLL-1, C-type lectin-like molecule-1.