Extended Data Figure 5. Fsh Ab Effects in Ovariectomized Mice.

Ovariectomized or sham-operated mice on normal chow injected with Fsh antibody (Ab) or goat IgG (200 or 400 µg/mouse/day to sham-operated and ovariectomized mice, respectively) (see Methods and Fig. 2). Shown are food intake and body weight (a) (n=5/group); plasma Fsh and estrogen (E₂) levels (plasma E₂ mostly undetectable after ovariectomy) (n=4 or 5/group) (b). Indirect calorimetry (metabolic cages) showing 24-hour respiratory quotient (RQ), resting EE (R-EE), active EE (A-EE), running distance (Wheel Meters), running speed (Wheel Speed), walking distance (Ped Meters), walking speed (Ped Speed) and food intake (n=4/group) (c). Absent effects of Fsh Ab or IgG on plasma glucose, total cholesterol, triglycerides or free fatty acids (n=4 or 5 mice/group) (d). Two-Tailed Student’s T-Test; **P≤0.01, or as shown.