Extended Data Table 1. Strategy to confirm datasets at the laboratories of Mount Sinai Bone Program and Maine Medical Center Research Institute.
The Fsh antibody was developed in 2008 in the laboratory of M.Z., and its effect on bone in ovariectomized mice was documented in around 2012 (refs 6, 7). Shortly after the recent discovery of its anti-adiposity action, M.Z. shared the antibody with C.J.R. in an attempt to synchronously replicate key findings in a different laboratory. Results were confirmed independently by investigators at the Mount Sinai Bone Program (M.Z., L.S., T.Y., and P. Liu) and Maine Medical Center Research Institute (C.J.R.), as well as, in specific instances where warranted, by external collaborators (H.M., S.H., C.B., Z.B., T.F.D., and X.E.G). This process involved free exchange of reagents and data and cross-confirmation at all levels to enhance rigor, transparency, and reproducibility. Furthermore, once the paper was accepted, the authors from both institutions went back to the Source Data to be doubly certain of their veracity, and in certain instances, the data were independently checked by a more distantly involved group (R.L.). Source Data are provided as Excel spreadsheets (for bar graphs) and a PDF file (for the raw immunoblot relating to Extended Data Fig. 1a). In this table, we attribute individual figure panels to specific principal investigators, which we hope will provide a new format for enhancing transparency and rigor.
| Figure | Primary PI | Collaborating PI |
|---|---|---|
| 1a | L.S. | |
| 1b | M.Z./P.Liu | C.B. |
| 1c | M.Z./P.Liu | X.E.G. |
| 2a | M.Z./P.Liu | C.B. |
| 2b | C.J.R. | |
| 2c | C.J.R. | |
| 3a | L.S./M.Z. | |
| 3b | T.Y. | |
| 3c | M.Z. | C.J.R. |
| 3d | M.Z./P.Liu | |
| 3e | M.Z./P.Liu | |
| 4a | M.Z./P.Liu | |
| 4b | M.Z./P.Liu | |
| 4c | M.Z./P.Liu | |
| 4d | L.S. | T.Y. |
| 5a | M.Z./P.Liu | |
| 5b | M.Z./P.Liu | |
| 5c | P.Liu | |
| Ext. 1a | T.Y. | |
| Ext. 1b | T.Y. | H.M. |
| Ext. 1c | H.M. | |
| Ext. 1d | S.H. | |
| Ext. 1e | M.Z. | C.J.R. |
| Ext. 1f | T.Y. | |
| Ext. 2a | C.J.R. | |
| Ext. 2b | C.J.R. | |
| Ext. 2c | C.J.R. | |
| Ext. 2d | C.J.R. | |
| Ext. 2e | C.J.R. | J.B.v.K. |
| Ext. 2f | T.Y. | |
| Ext. 2g | T.Y | |
| Ext. 2h | C.J.R. | |
| Ext. 2i | C.J.R. | |
| Ext. 2j | L.S. | |
| Ext. 2k | L.S. | |
| Ext. 2l | L.S. | |
| Ext 2m | C.J.R. | J.B.v.K. |
| Ext. 3a | L.S. | |
| Ext. 3b | M.Z./P.Liu | C.B. |
| Ext. 3c | M.Z./P.Liu | X.E.G. |
| Ext. 4a | M.Z./P.Liu | C.B. |
| Ext. 4b | M.Z./P.Liu | X.E.G. |
| Ext. 4c | M.Z./P.Liu | |
| Ext. 5a | L.S. | |
| Ext. 5b | L.S. | T.Y. |
| Ext. 5c | C.J.R. | |
| Ext. 5d | L.S. | C.J.R. |
| Ext. 6a | L.S. | |
| Ext. 6b | M.Z./P.Liu | C.B. |
| Ext. 6c | M.Z./P.Liu | X.E.G. |
| Ext. 6d | L.S. | |
| Ext. 6e | M.Z./P.Liu | C.B. |
| Ext. 6f | M.Z./P.Liu | X.E.G. |
| Ext. 6g | C.J.R. | |
| Ext. 7a | L.S. | Z.B. |
| Ext. 7b | L.S. | |
| Ext. 7c | L.S. | |
| Ext. 8a | L.S. | T.Y. |
| Ext. 8b | L.S. | T.Y. |
| Ext. 9a | L.S. | |
| Ext. 9b | L.S. | |
| Ext. 9c | M.Z./P.Liu | C.B. |
| Ext. 9d | M.Z./P.Liu | X.E.G. |
| Ext. 9e | P.Liu |