Fig. 4.

Molecular targets of Orm1 in mouse regenerating livers. Microarray analysis (GSE83733) was performed in control siRNA (siCtl) and Orm1 siRNA (siOrm1)-injected mouse livers at 48 h post-PH (n = 2). (a) Heatmap visualization of 188 differentially expressed genes with a fold change of > 2 in the livers between groups of mice receiving siOrm1 or siCtl. (b) Top five associated signaling pathways performed using SEA analysis in GeneSpring GX13. (c) The top diseases or functions annotation and (d) top canonical pathway analysis performed in IPA platform. The pathways were ranked according to their-log10 of P values. The ratio indicates the number of enriched genes of interest relative to the total number of genes associated with that pathway in the IPA database. (e) Gene expression levels of MCM2, MCM4 and MCM6 involved in the enriched “cell cycle control of chromosomal replication” singling pathway in whole-liver tissues before (PH 0 h) and at 48 h post-PH (PH 48 h) were verified using RT-PCR and presented as fold change compared to PH 0 h (n = 4). The data were presented in a Box-and-Whisker plot. *P < 0.05 assessed using the Mann-Whitney U test or two-tailed Student's t-test.