Fig. 2.

MDM2i prevents induction of polyploidy by AURKAi. (a) Proliferation analysis using the BRDU incorporation assay. Cells were treated with 10 μM nutlin-3a (MDM2i) ± 1 μM alisertib (AURKAi) for 3 days. Gates show percent of BRDU-positive cells in relation to DNA content. Numbers next to gates indicate the percentage of cells. (b) Quantified results of the BRDU analysis shown in (a). Raw data, means, SEM, and the results of ANOVA with Tukey's comparison are indicated. (c) Western blot analysis of indicated cell cycle regulators in cells treated as in (a). Experiments were repeated at least 3 times with consistent results.