Fig. 6.

Ad-Lkb1 induces hindlimb paralysis through activation of mTOR signaling. (a and b) The mRNA and protein expression of Lkb1 and mTOR in BAT from WT (N = 4) and DK (N = 5) mice (8-month-old), **P < 0.01. (c) Representative images and foot-print of WT, Lkb1 KO and Lkb1/mTOR DK mouse (10-month-old). Fore-paws were immersed in red ink and hind limbs were immersed in blue ink. (d) Relative mRNA levels of pro-inflammatory factors and chemokines of BAT in WT (N = 3), KO (N = 3) and DK (N = 3) groups. (e) CD68 immunostaining of sciatic nerve from WT, KO and DK mice, N = 3 pairs (three random pictures were counted from each mice), scale bar: 100 μm. (f and g) sciatic nerve TEM images (f) and axon size distribution (g) of WT, KO and DK mice (male, 9-month-old), N = 3 pairs. (h and i) Numbers of normal, abnormal myelinated axons per area (h) and percentages of abnormal axons (i), N = 3 pairs. (j) CAP amplitudes of WT (N = 4), KO (N = 7) and DK (N = 6) from 8–10-month old mice. The number of samples are biological replicates. Error bars: S.E.M., *P < 0.05, **P < 0.01, KO compared with WT mice; ^#P < 0.05, ^##P < 0.01, DK compared with KO mice (Student's t-test).