Fig. 3.

Effects of ivermectin and nifuroxazide on mitochondrial ATP levels under hypoxia in primary cardiomyocytes. (a) A schematic of mito-ATeam knock-in animals for the isolation of primary cardiomyocytes. (b) Representative images of mito-ATeam in primary cardiomyocytes isolated from mito-ATeam knock-in neonatal hearts stained for mitochondria (red) and nuclei (blue); mito-ATeam is in green. Scale bar, 10 μm. (c) Quantified FRET ratios of mito-ATeam knock-in primary cardiomyocytes pre-treated with vehicle (V), ivermectin (2 or 5 μM; I2 or I5), or nifuroxazide (10 μM, N10) for 24 h followed by hypoxia/reoxygenation. Data are presented as means ± SD (n = 3 biologically independent samples).