Fig. 4.

Identification of importin β as a potential molecular target of ivermectin. (a) Expression levels of genes involved in ivermectin action were determined by RNA sequencing (RNA-seq) of HL-1 cardiomyocytes. Data are presented as means ± SD (n = 3 biologically independent samples). FPKM, fragments per kilobase of transcript per million mapped reads. (b) mito-ATeam stable HL-1 cardiomyocytes were treated with vehicle, the importin β inhibitor importazole (3 or 10 μM), or the P2X4 positive allosteric modulator 2-meSATP (10 or 25 μM) for 24 h. Quantified FRET ratios of mito-ATeam under hypoxia/reoxygenation are shown. Data are presented as means ± SD (n = 3 biologically independent samples).