Figure 2.

The exogenous effects of DKK-3 protein were investigated in KPK1 cells in terms of the downstream targets of Wnt/β-catenin signaling. Cell samples were obtained prior to treatment and at 6, 24, 48 and 72 h after treatment. The expression of TCF1 and c-Myc was examined via western blot analysis as the key effectors of the signaling pathway. β-actin was used as the loading control. DKK, Dickkopf-related protein; REIC, reduced expression in immortalized cells; TCF1, transcription factor 1.