Figure 4.

It was evaluated whether exogenous DKK-3 protein affects LRP6-mediated Wnt/β-catenin signaling. Following depletion of LRP6 expression by siRNA, the exogenous effects of DKK-3 were investigated in KPK1 cells in terms of the downstream targets of Wnt signaling. Cell samples were obtained prior to treatment and at 6, 24, 48 and 72 h after treatment. The expression of p-β-catenin and c-Myc was examined via western blot analysis as the key effectors of the signaling pathway. β-actin was used as the loading control. DKK, Dickkopf-related protein; p-, phosphorylated; LRP6, low-density lipoprotein receptor-related protein 6; REIC, reduced expression in immortalized cells; siRNA, small interfering RNA.