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Measurement of IDO enzymatic activity in immortalized biliary epithelial cell supernatants. Enzymatic activity was quantified by the colorimetric assay for kynurenine, in the presence of IFN-γ (20 ng/ml) for 0 to 120 h. IFN-γ, interferon-γ. The difference was significant (***P<0.05) between the supernatant of stimulated and unstimulated (control) cells.
