Figure 1.

Expression of PKN3 mRNA and suppression of PKN3 mRNA by transfection with PKN3 small interfering RNA (siRNA) in human and mouse tumor cell lines. (A) The expression levels of PKN3 mRNA in human and mouse tumor cell lines were analyzed by reverse transcription-polymerase chain reaction (RT-PCR). (B) Human MDA-MB-231, PANC-1 and HeLa cells, and (C) mouse LLC and Colon 26 cells were transfected with 50 nM Cont siRNA or PKN3 siRNA using Lipofectamine RNAiMax reagent, and the expression levels of PKN3 mRNA in the cells were analyzed by RT-quantitative PCR (RT-qPCR). Each result represents the mean ± standard deviation (n=3). PKN3, protein kinase N3; siRNA, small interfering RNA; Cont, control.