Figure 5.

Change in vascular vessels in liver metastasized MDA-MB-231 tumors after combination therapy of PKN3 siRNA and DXR. (A) Immunostaining for endothelial cells in tumors shown in Fig. 4. White signals indicate CD31-positive endothelial cells. Scale bar, 100 µm. (B) Mouse VEGF mRNA level in livers with MDA-MB-231 tumor metastasis. Each result represents the mean (n=2 for Cont siRNA) or the mean ± standard deviation (n=4). *P<0.05, **P<0.01, compared with no treatment. PKN3, protein kinase N3; Cont, control; siRNA, small interfering RNA; DXR, doxorubicin.