Table 1. Oxygen-limited metabolism in the methanotroph Methylomicrobium buryatense 5GB1C Bioreactor data summary.
Experiment ID | FM86 | FM87 | FM88 | FM90 |
---|---|---|---|---|
Strain | 5GB1 WT | 5GB1 WT | 5GB1 ΔCyt aa3 | 5GB1 ΔCyt aa3 |
Dilution rate (h−1) or growth rate | 0.036 | 0.035 | 0.034 | 0.035 |
Cell density (gCDW L−1) | 0.32 | 0.28 | 0.25 | 0.21 |
Dissolved oxygen (mg L−1) | 0.22 | 0.25 | 0.25 | 0.26 |
Oxygen uptake (mmol h−1) | 0.92 | 0.94 | 0.95 | 0.94 |
Methane uptake (mmol h−1) | 0.86 | 0.88 | 0.88 | 0.89 |
Specific oxygen uptake (mmol gCDW−1 h−1) | 2.9 | 3.1 | 3.9 | 4.5 |
Specific methane uptake (mmol gCDW−1 h−1) | 2.7 | 2.9 | 3.6 | 4.2 |
O2/CH4 uptake ratio | 1.1 | 1.1 | 1.1 | 1.1 |
Inlet gas composition | 20% CH4, 5%O2, 75%N2 | 20% CH4, 5%O2, 75%N2 | 20% CH4, 5%O2, 75%N2 | 20% CH4, 5%O2, 75%N2 |
Formate (μmol gCDW−1) | 479.1 | 203.1 | 1216.3 | 2995.2 |
Acetate (μmol gCDW−1) | 30.2 | 39.2 | 85.7 | 81.0 |